Reinigung, biochemische und biophysikalische Charakterisierung einer sauren α-Galactosidase aus den Samen von Annona squamosa (Puddingapfel)

 

Alpha-Galactosidase ist eine Exoglycosidase, die α-D-Galactose spaltet und zahlreiche Anwendungen in der Medizin-, Biotechnologie-, Lebensmittel- und Pharmaindustrie hat. In dieser Studie wurde eine niedermolekulare saure α-Galactosidase aus den Samen von Puddingapfel identifiziert. Die Reinigung von α-Galactosidase aus dem Rohextrakt entfetteter Samen wurde unter Verwendung von Ammoniumsulfatfraktionierung, hydrophober Wechselwirkung und Gelfiltrationschromatographietechniken erreicht. Die gereinigte Puddingapfel-α-Galactosidase (CaG) wanderte als einzelne Bande in nativer PAGE entsprechend einem Molekulargewicht von ~ 67 kDa und gespaltenen chromogenen, fluorogenen und natürlichen Substraten.

Es wurde gefunden, dass CaG ein Heterodimer mit Untereinheitenmassen von 40 und 30 kDa ist. Die kinetischen Parameter wie KM und Vmax betrugen mit p-Nitrophenyl-α-D-galactopyranosid 0,67 mM bzw. 1,5 U / mg. Galactose, Methyl-α-D-Galactopyranosid und D-Galacturonsäure hemmten die CaG-Aktivität im gemischten Modus. Die CD-Spektralanalyse im fernen UV-Bereich zeigte, dass gereinigtes CaG in seiner Sekundärstruktur überwiegend als Helix (35%), Beta-Faltblätter (16,3%) und Zufallsspulen (32,3%) vorliegt. Diese biochemischen und biophysikalischen Eigenschaften von CaG führen zum Verständnis seiner Primärsequenz und seiner Glykanstrukturen, die schließlich seine neuartigen physiologischen Rollen in Pflanzen und potenziellen industriellen Anwendungen definieren werden.

In diesem Artikel wird der Einfluss von Faktoren auf die Produktinnovation untersucht, die auf der Transformation und Nutzung von Wissen aus den organisationsinternen Beziehungen eines Unternehmens beruhen. Insbesondere analysiert dieses Papier den Einfluss des intraorganisatorischen Sozialkapitals (SC) (d. H. Bestehend aus strukturellen, relationalen und kognitiven Dimensionen) auf die realisierte Absorptionskapazität (RACAP). Darüber hinaus wird die Vermittlerrolle von RACAP in Bezug auf die Beziehung zwischen internem SC und Produktinnovation analysiert. Basierend auf einer Stichprobe von Unternehmen aus der spanischen biotechnologischen und pharmazeutischen Industrie wurden zwei Hypothesen unter Verwendung eines Strukturgleichungsmodells und der PLS-Technik (Partial Least Squares) getestet.

Die Ergebnisse stützen beide Hypothesen, was darauf hindeutet, dass die Entwicklung starker und enger Verbindungen, die auf einem gemeinsamen Verständnis und Vertrauen der Unternehmensmitglieder beruhen, das Unternehmen dazu veranlasst, dynamische Fähigkeiten zur Transformation und Nutzung von extern erworbenem Wissen zu entwickeln, was Innovationen auf der Grundlage der Entwicklung neuer Produkte fördert. Forschungsgrenzen, Implikationen und zukünftige Forschung werden auch von den Autoren des Papiers diskutiert.

 

Intensive Bitterkeit von Molekülen: Maschinelles Lernen zur Beschleunigung der Wirkstoffentdeckung

Die Arzneimittelentwicklung ist ein langer, teurer und mehrstufiger Prozess, der darauf abzielt, sichere Arzneimittel mit hoher Wirksamkeit zu erzielen. Eine entscheidende Voraussetzung für den Abschluss des Medikationsschemas für orale Medikamente, insbesondere für pädiatrische und geriatrische Bevölkerungsgruppen, ist das Erreichen eines Geschmacks, der die Compliance nicht behindert. Derzeit wird der aversive Geschmack von Arzneimitteln in späten Stadien klinischer Studien getestet. Dies kann zu einer Neuformulierung führen, die möglicherweise dazu führt, dass mehr Tiere für zusätzliche Toxizitätsversuche verwendet werden, die finanziellen Kosten steigen und die Markteinführung verzögert wird.

Hier präsentieren wir BitterIntense, ein Werkzeug für maschinelles Lernen, das Moleküle aufgrund ihrer chemischen Struktur in “sehr bitter” oder “nicht sehr bitter” klassifiziert. Das Modell, das auf chemisch verschiedenen Verbindungen trainiert wurde, weist bei mehreren Testsätzen eine Genauigkeit von über 80% auf. Unsere Ergebnisse legen nahe, dass etwa 25% der Arzneimittel als sehr bitter vorhergesagt werden, wobei die Prävalenz (~ 40%) bei COVID19-Arzneimittelkandidaten und bei mikrobiellen Naturstoffen noch höher ist. Es wird vorausgesagt, dass nur ~ 10% der toxischen Moleküle stark bitter sind, und es wird auch vermutet, dass intensive Bitterkeit nicht mit der Hepatotoxizität von Arzneimitteln korreliert.

Sehr bittere Verbindungen können jedoch kardiotoxischer sein als nicht sehr bittere Verbindungen, die signifikant niedrigere QPlogHERG-Werte besitzen. BitterIntense ermöglicht eine schnelle und einfache Vorhersage der starken Bitterkeit von Verbindungen, die für die Lebensmittel-, Pharma- und Biotechnologieindustrie von Interesse sind. Wir schätzen, dass die Implementierung von BitterIntense oder ähnlichen Tools zu Beginn des Arzneimittelentdeckungsprozesses zu einer Verringerung der Verzögerungen, des Tiergebrauchs und der finanziellen Gesamtbelastung führen kann.

Empfehlungen zur Straffung und Standardisierung der Machbarkeitsbewertungen für Standorte für klinische Studien: Eine ASCO-Forschungserklärung

Machbarkeitsbewertungen (FAs) sind wichtig, um die Standortfähigkeiten für die Durchführung klinischer Studien und ihre Eignung für bestimmte Studien zu ermitteln. Derzeitige FA-Methoden, die von Sponsoren von Biotechnologie- und Pharmaversuchen (Biotech-Pharma) und Auftragsforschungsorganisationen (CROs) verwendet werden, sind jedoch kostspielig, ineffizient, unnötig belastend und ressourcenintensiv. Diese Methoden verzögern den Start der Studie, wirken als Hindernis für die Teilnahme am Standort und verringern letztendlich den rechtzeitigen Zugang der Patienten zu klinischen Studien und neuartigen Behandlungen.
Methoden: Eine ASCO-Task Force wurde einberufen, um die spezifischen Belastungen und Herausforderungen der FA zu bewerten und Empfehlungen zur Verbesserung ihrer Effizienz und Effektivität zu entwickeln. Stakeholder (einschließlich Versuchsstandorte, Biotech-Pharma-Sponsoren und CROs) gaben Einblicke in Herausforderungen und boten Lösungen durch zwei Umfragen und ein persönliches Treffen an.

Die Task Force nutzte das Feedback, um Konsensempfehlungen zur Verbesserung der FAs für klinische Studien in der Onkologie zu formulieren.
Ergebnisse: Drei wichtige Empfehlungen wurden für die Anwendung in allen von Biotech-Pharma gesponserten Studien ermittelt: (1) Implementierung eines optimierten und einheitlichen FA-Prozesses in allen Studien und Sponsoren; (2) Fragen minimieren und standardisieren; und (3) Nutzung von Technologie zur Zentralisierung von FAs, Erleichterung der Kommunikation und Reduzierung von Redundanzen.
Schlussfolgerung: Es ist dringend erforderlich, den derzeitigen FA-Prozess zu verbessern, der kostspielig, inkonsistent, ineffizient, arbeitsintensiv und von ungewisser Wirksamkeit ist.

 

Congo Red Solution
CRA500	ScyTek Laboratories	500 ml	EUR 106

Congo Red Solution
CRA999	ScyTek Laboratories	1000 ml	EUR 143

Congo Red (C.I. 22120)
GT5674-100G	Glentham Life Sciences	100 g	EUR 62

Congo Red (C.I. 22120)
GT5674-250G	Glentham Life Sciences	250 g	EUR 86

Congo Red (C.I. 22120)
GT5674-25G	Glentham Life Sciences	25 g	EUR 45

FD Congo Red Solution™
PS108	FD Neurotechnologies	500 ml	EUR 237

Congo Red "high purity grade"
80028	Biotium	100MG	EUR 172
Description: Minimum order quantity: 1 unit of 100MG

Amyloid Stain Kit (Congo Red)
AMY-1	ScyTek Laboratories	1 kit(s)	EUR 165

Amyloid Stain Kit (Congo Red)
AMY-2	ScyTek Laboratories	1 kit(s)	EUR 109

Congo Red *UltraPure grade* *CAS 573-58-0*
23056	AAT Bioquest	100 mg	EUR 115

Paper 2.25"x50 feet
B4000-PA	BenchMark	1 PC	EUR 342.53

thermal paper 112mm/25m
AM112	Consort	ea	EUR 24

UPP-110HG Print paper
2140913	Atto	1unit	EUR 356

Weighing Paper, 3X3, 200Sheets/Bag
WT111	Bio Basic	1PK, 200UNIT	EUR 57.29

Weighing Paper, 4X4, 100Sheets/Bag
WT112	Bio Basic	1PK, 100UNIT	EUR 57.83

Weighing Paper, 6X6, 100Sheets/Bag
WT113	Bio Basic	1PK, 100UNIT	EUR 60.44

Blotting Paper, 10X15cm, 100 Sheets
PP3322	Bio Basic	1PK, 100UNIT	EUR 105.68

Blotting Paper, 15X15cm, 100 Sheets
PP3323	Bio Basic	1PK, 100UNIT	EUR 117.04

Blotting Paper, 20X20cm, 100 Sheets
PP3324	Bio Basic	1PK, 100UNIT	EUR 166.4

Chymase reagent
30C-CP1129	Fitzgerald	5 units	EUR 2185
Description: Purified native Human Chymase reagent

Traut's Reagent
2330-1000	Biovision		EUR 349

Traut's Reagent
2330-500	Biovision		EUR 207

MTS Reagent
2808-1000	Biovision		EUR 990

MTS Reagent
2808-250	Biovision		EUR 365

MTT Reagent
2809-1G	Biovision		EUR 180

MTT Reagent
2809-5G	Biovision		EUR 544

BOP reagent
5-02141	CHI Scientific	25g	Ask for price

BOP reagent
5-02142	CHI Scientific	100g	Ask for price

Bradford reagent
BDE641	Bio Basic	100ml	EUR 61.01

Beaucage reagent
HY-100951	MedChemExpress	10mM/1mL	EUR 126

BOP reagent
A7015-100000	ApexBio	100 g	EUR 200
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

BOP reagent
A7015-25000	ApexBio	25 g	EUR 113
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

Bluing Reagent
BRT030	ScyTek Laboratories	30 ml	EUR 60

Bluing Reagent
BRT125	ScyTek Laboratories	125 ml	EUR 63

Bluing Reagent
BRT3800	ScyTek Laboratories	1 Gal.	EUR 184

Bluing Reagent
BRT500	ScyTek Laboratories	500 ml	EUR 76

Bluing Reagent
BRT999	ScyTek Laboratories	1000 ml	EUR 88

HAMA blocking reagent
85R-1001	Fitzgerald	1 gram	EUR 1974
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA

HAMA blocking reagent
85R-1001P	Fitzgerald	1 gram	EUR 2190
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA

HAMA blocking reagent
85R-1003	Fitzgerald	1 gram	EUR 1974
Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests

HAMA blocking reagent
85R-1014	Fitzgerald	50 mg	EUR 192
Description: HAMA blocking reagent for use in assays specific for clinical false positive samples

HAMA blocking reagent
85R-1025	Fitzgerald	50 mg	EUR 192
Description: HAMA blocking reagent for use in immunoassays

HAMA blocking reagent
85R-1026	Fitzgerald	50 mg	EUR 192
Description: HAMA blocking reagent for use in immunoassays

ExFect2000 Transfection Reagent
T202-01	Vazyme	0.5 ml	EUR 227

ExFect2000 Transfection Reagent
T202-02	Vazyme	1 ml	EUR 316

ExFect2000 Transfection Reagent
T202-03	Vazyme	5 ml	EUR 1052

BSA (Reagent Grade)
30-AB79	Fitzgerald	1 kg	EUR 1552
Description: Reagent Grade Bovine Serum Albumin (99% pure)

BSA (Reagent Grade)
30-AB81	Fitzgerald	200 grams	EUR 476
Description: Reagent Grade Sulphydryl Blocked BSA (99% pure)

BODIPY-Acetylene Reagent
2594-1	Biovision		EUR 207

BODIPY-Acetylene Reagent
2594-5	Biovision		EUR 675

Biotin reagent (HRP)
65C-CE0202	Fitzgerald	5 mg	EUR 244
Description: HRP conjugated biotin labelling reagent

Bradford Dye Reagent
0209R	AthenaES	100 ml	EUR 131

BCA Reagent, 16ML
C144-16ML	Arbor Assays	16ML	EUR 163

Dissociation Reagent, 1ML
X017-1ML	Arbor Assays	1ML	EUR 109

Dissociation Reagent, 25ML
X017-25ML	Arbor Assays	25ML	EUR 258

Dissociation Reagent, 5ML
X017-5ML	Arbor Assays	5ML	EUR 122

Dissociation Reagent, 1ML
X058-1ML	Arbor Assays	1ML	EUR 73

Dissociation Reagent, 5ML
X058-5ML	Arbor Assays	5ML	EUR 109

Biolipidure-1002-Reagent
Biolipidure-1002-10	Cusabio	10mL	EUR 196
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Convoy? Transfection Reagent
1110-1ml	ACTGene		EUR 341

Griess Reagent Kit
30100	Biotium	1KIT	EUR 149
Description: Minimum order quantity: 1 unit of 1KIT

PhosphoBlocker Blocking Reagent
AKR-103	Cell Biolabs	1L	EUR 328
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

PhosphoBlocker Blocking Reagent
AKR-104	Cell Biolabs	4L	EUR 711
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

Girard's reagent T
20-abx184099	Abbexa	EUR 203.00 EUR 314.00	100 g 500 g

FcR blocking Reagent
20-abx290024	Abbexa	EUR 377.00 EUR 516.00	200 tests 400 tests

Detection Reagent A
abx296004-120ul	Abbexa	120 ul	EUR 321

Mycoplasma Prevention Reagent
20-abx298005	Abbexa	EUR 203.00 EUR 286.00	1 ml 5 ml

EL Transfection Reagent
20-abx098880	Abbexa	EUR 384.00 EUR 537.00	0.75 ml 1.5 ml

Mycoplasma Prevention Reagent
20-abx098886	Abbexa	EUR 425.00 EUR 509.00	1 ml 5 ml

TissueDigest Reagent, 20X
T101	Insitus Biotechnologies	10ml	EUR 210

HighGene transfection reagent
RM09014	Abclonal	1000μl	EUR 270

LP4K Transfection Reagent
LP4K	GenTarget	1.0 ml / vial	EUR 304
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types.

n-Heptane Reagent
HC5400	Bio Basic	1L	EUR 79

Tri-RNA Reagent
FATRR-001	Favorgen	100ml	EUR 236

Tri-RNA Reagent
FATRR-002	Favorgen	50ml	EUR 176

Tri-RNA Reagent
FATRR-003	Favorgen	450ml	EUR 645

DTT (Cleland's reagent)
DB0058	Bio Basic	5g	EUR 84.8

DTNB (Ellman's Reagent)
DB0113	Bio Basic	5g	EUR 97.85

Ethyl acetate Reagent
EC4600	Bio Basic	1L	EUR 79

Biolipidure-1002-Reagent
Biolipidure-1002-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-103-Reagent
Biolipidure-103-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-103-Reagent
Biolipidure-103-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1201-Reagent
Biolipidure-1201-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1201-Reagent
Biolipidure-1201-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1301-Reagent
Biolipidure-1301-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1301-Reagent
Biolipidure-1301-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-203-Reagent
Biolipidure-203-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-203-Reagent
Biolipidure-203-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-206-Reagent
Biolipidure-206-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-206-Reagent
Biolipidure-206-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-405-Reagent
Biolipidure-405-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-405-Reagent
Biolipidure-405-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-502-Reagent
Biolipidure-502-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-502-Reagent
Biolipidure-502-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-702-Reagent
Biolipidure-702-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-702-Reagent
Biolipidure-702-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-802-Reagent
Biolipidure-802-10	Biolipidure	10mL	EUR 196
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-802-Reagent
Biolipidure-802-100	Biolipidure	100mL	EUR 1223
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Alcohol, Reagent (70%)
EAS500	ScyTek Laboratories	500 ml	EUR 79

Alcohol, Reagent (70%)
EAS999	ScyTek Laboratories	1000 ml	EUR 101

PureFection Transfection Reagent
LV750A-1	SBI	1 ml	EUR 359

Printer paper for AB-2270, 10/pk
3511065	Atto	2unit	EUR 363

Extra paper rolls for MR9600-TP printer
MR9600-PA	BenchMark	1 PC	EUR 86.75

RED Antibody
ABD4352	Lifescience Market	100 ug	EUR 438

Neutral Red
G035	ABM	5 x 1.0ml	EUR 125

Ruthenium Red
2490-100	Biovision		EUR 131

Ruthenium Red
2490-500	Biovision		EUR 370

RED antibody
70R-13184	Fitzgerald	100 ul	EUR 457
Description: Affinity purified Rabbit polyclonal RED antibody

RED Antibody
DF4352	Affbiotech	200ul	EUR 304
Description: RED Antibody detects endogenous levels of total RED.

Nile Red
60029	Biotium	10MG	EUR 125
Description: Minimum order quantity: 1 unit of 10MG

RED Antibody
20-abx249463	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg

Cresol Red
GT0793-100G	Glentham Life Sciences	100 g	EUR 78

Cresol Red
GT0793-25G	Glentham Life Sciences	25 g	EUR 43

Nile Red
GT2331-1G	Glentham Life Sciences	1 g	EUR 174

Nile Red
GT2331-250MG	Glentham Life Sciences	250 mg	EUR 87

Chlorophenol Red
GT3722-10G	Glentham Life Sciences	10 g	EUR 78

Chlorophenol Red
GT3722-25G	Glentham Life Sciences	25 g	EUR 126

Chlorophenol Red
GT3722-50G	Glentham Life Sciences	50 g	EUR 198

Chlorophenol Red
GT3722-5G	Glentham Life Sciences	5 g	EUR 59

Pyrogallol Red
GT7202-10G	Glentham Life Sciences	10 g	EUR 158

Pyrogallol Red
GT7202-1G	Glentham Life Sciences	1 g	EUR 54

Pyrogallol Red
GT7202-5G	Glentham Life Sciences	5 g	EUR 110

Bromophenol Red
GT7578-10G	Glentham Life Sciences	10 g	EUR 142

Alle Beteiligten können von der Umsetzung dieser Empfehlungen profitieren, die darauf abzielen, die Belastungen zu minimieren und sicherzustellen, dass mehr Studienorte und Patienten rechtzeitig Zugang zu klinischen Onkologie-Studien haben. Um eine sinnvolle Wirkung zu erzielen, ist die Annahme und konsequente Umsetzung dieser Empfehlungen in allen Studien, Sponsoren, CROs und Standorten von entscheidender Bedeutung.