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Reinigung, biochemische und biophysikalische Charakterisierung einer sauren α-Galactosidase aus den Samen von Annona squamosa (Puddingapfel)

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Reinigung, biochemische und biophysikalische Charakterisierung einer sauren α-Galactosidase aus den Samen von Annona squamosa (Puddingapfel)

 

Alpha-Galactosidase ist eine Exoglycosidase, die α-D-Galactose spaltet und zahlreiche Anwendungen in der Medizin-, Biotechnologie-, Lebensmittel- und Pharmaindustrie hat. In dieser Studie wurde eine niedermolekulare saure α-Galactosidase aus den Samen von Puddingapfel identifiziert. Die Reinigung von α-Galactosidase aus dem Rohextrakt entfetteter Samen wurde unter Verwendung von Ammoniumsulfatfraktionierung, hydrophober Wechselwirkung und Gelfiltrationschromatographietechniken erreicht. Die gereinigte Puddingapfel-α-Galactosidase (CaG) wanderte als einzelne Bande in nativer PAGE entsprechend einem Molekulargewicht von ~ 67 kDa und gespaltenen chromogenen, fluorogenen und natürlichen Substraten.

Es wurde gefunden, dass CaG ein Heterodimer mit Untereinheitenmassen von 40 und 30 kDa ist. Die kinetischen Parameter wie KM und Vmax betrugen mit p-Nitrophenyl-α-D-galactopyranosid 0,67 mM bzw. 1,5 U / mg. Galactose, Methyl-α-D-Galactopyranosid und D-Galacturonsäure hemmten die CaG-Aktivität im gemischten Modus. Die CD-Spektralanalyse im fernen UV-Bereich zeigte, dass gereinigtes CaG in seiner Sekundärstruktur überwiegend als Helix (35%), Beta-Faltblätter (16,3%) und Zufallsspulen (32,3%) vorliegt. Diese biochemischen und biophysikalischen Eigenschaften von CaG führen zum Verständnis seiner Primärsequenz und seiner Glykanstrukturen, die schließlich seine neuartigen physiologischen Rollen in Pflanzen und potenziellen industriellen Anwendungen definieren werden.

In diesem Artikel wird der Einfluss von Faktoren auf die Produktinnovation untersucht, die auf der Transformation und Nutzung von Wissen aus den organisationsinternen Beziehungen eines Unternehmens beruhen. Insbesondere analysiert dieses Papier den Einfluss des intraorganisatorischen Sozialkapitals (SC) (d. H. Bestehend aus strukturellen, relationalen und kognitiven Dimensionen) auf die realisierte Absorptionskapazität (RACAP). Darüber hinaus wird die Vermittlerrolle von RACAP in Bezug auf die Beziehung zwischen internem SC und Produktinnovation analysiert. Basierend auf einer Stichprobe von Unternehmen aus der spanischen biotechnologischen und pharmazeutischen Industrie wurden zwei Hypothesen unter Verwendung eines Strukturgleichungsmodells und der PLS-Technik (Partial Least Squares) getestet.

Die Ergebnisse stützen beide Hypothesen, was darauf hindeutet, dass die Entwicklung starker und enger Verbindungen, die auf einem gemeinsamen Verständnis und Vertrauen der Unternehmensmitglieder beruhen, das Unternehmen dazu veranlasst, dynamische Fähigkeiten zur Transformation und Nutzung von extern erworbenem Wissen zu entwickeln, was Innovationen auf der Grundlage der Entwicklung neuer Produkte fördert. Forschungsgrenzen, Implikationen und zukünftige Forschung werden auch von den Autoren des Papiers diskutiert.

 

Intensive Bitterkeit von Molekülen: Maschinelles Lernen zur Beschleunigung der Wirkstoffentdeckung

Die Arzneimittelentwicklung ist ein langer, teurer und mehrstufiger Prozess, der darauf abzielt, sichere Arzneimittel mit hoher Wirksamkeit zu erzielen. Eine entscheidende Voraussetzung für den Abschluss des Medikationsschemas für orale Medikamente, insbesondere für pädiatrische und geriatrische Bevölkerungsgruppen, ist das Erreichen eines Geschmacks, der die Compliance nicht behindert. Derzeit wird der aversive Geschmack von Arzneimitteln in späten Stadien klinischer Studien getestet. Dies kann zu einer Neuformulierung führen, die möglicherweise dazu führt, dass mehr Tiere für zusätzliche Toxizitätsversuche verwendet werden, die finanziellen Kosten steigen und die Markteinführung verzögert wird.

Hier präsentieren wir BitterIntense, ein Werkzeug für maschinelles Lernen, das Moleküle aufgrund ihrer chemischen Struktur in “sehr bitter” oder “nicht sehr bitter” klassifiziert. Das Modell, das auf chemisch verschiedenen Verbindungen trainiert wurde, weist bei mehreren Testsätzen eine Genauigkeit von über 80% auf. Unsere Ergebnisse legen nahe, dass etwa 25% der Arzneimittel als sehr bitter vorhergesagt werden, wobei die Prävalenz (~ 40%) bei COVID19-Arzneimittelkandidaten und bei mikrobiellen Naturstoffen noch höher ist. Es wird vorausgesagt, dass nur ~ 10% der toxischen Moleküle stark bitter sind, und es wird auch vermutet, dass intensive Bitterkeit nicht mit der Hepatotoxizität von Arzneimitteln korreliert.

Sehr bittere Verbindungen können jedoch kardiotoxischer sein als nicht sehr bittere Verbindungen, die signifikant niedrigere QPlogHERG-Werte besitzen. BitterIntense ermöglicht eine schnelle und einfache Vorhersage der starken Bitterkeit von Verbindungen, die für die Lebensmittel-, Pharma- und Biotechnologieindustrie von Interesse sind. Wir schätzen, dass die Implementierung von BitterIntense oder ähnlichen Tools zu Beginn des Arzneimittelentdeckungsprozesses zu einer Verringerung der Verzögerungen, des Tiergebrauchs und der finanziellen Gesamtbelastung führen kann.

Reinigung, biochemische und biophysikalische Charakterisierung einer sauren α-Galactosidase aus den Samen von Annona squamosa (Puddingapfel)

Empfehlungen zur Straffung und Standardisierung der Machbarkeitsbewertungen für Standorte für klinische Studien: Eine ASCO-Forschungserklärung

Machbarkeitsbewertungen (FAs) sind wichtig, um die Standortfähigkeiten für die Durchführung klinischer Studien und ihre Eignung für bestimmte Studien zu ermitteln. Derzeitige FA-Methoden, die von Sponsoren von Biotechnologie- und Pharmaversuchen (Biotech-Pharma) und Auftragsforschungsorganisationen (CROs) verwendet werden, sind jedoch kostspielig, ineffizient, unnötig belastend und ressourcenintensiv. Diese Methoden verzögern den Start der Studie, wirken als Hindernis für die Teilnahme am Standort und verringern letztendlich den rechtzeitigen Zugang der Patienten zu klinischen Studien und neuartigen Behandlungen.
Methoden: Eine ASCO-Task Force wurde einberufen, um die spezifischen Belastungen und Herausforderungen der FA zu bewerten und Empfehlungen zur Verbesserung ihrer Effizienz und Effektivität zu entwickeln. Stakeholder (einschließlich Versuchsstandorte, Biotech-Pharma-Sponsoren und CROs) gaben Einblicke in Herausforderungen und boten Lösungen durch zwei Umfragen und ein persönliches Treffen an.

Die Task Force nutzte das Feedback, um Konsensempfehlungen zur Verbesserung der FAs für klinische Studien in der Onkologie zu formulieren.
Ergebnisse: Drei wichtige Empfehlungen wurden für die Anwendung in allen von Biotech-Pharma gesponserten Studien ermittelt: (1) Implementierung eines optimierten und einheitlichen FA-Prozesses in allen Studien und Sponsoren; (2) Fragen minimieren und standardisieren; und (3) Nutzung von Technologie zur Zentralisierung von FAs, Erleichterung der Kommunikation und Reduzierung von Redundanzen.
Schlussfolgerung: Es ist dringend erforderlich, den derzeitigen FA-Prozess zu verbessern, der kostspielig, inkonsistent, ineffizient, arbeitsintensiv und von ungewisser Wirksamkeit ist.

 

Congo Red Solution
CRA500 500 ml
EUR 106

Congo Red Solution
CRA999 1000 ml
EUR 143

Congo Red (C.I. 22120)
GT5674-100G 100 g
EUR 62

Congo Red (C.I. 22120)
GT5674-250G 250 g
EUR 86

Congo Red (C.I. 22120)
GT5674-25G 25 g
EUR 45

FD Congo Red Solution™
PS108 500 ml
EUR 237

Congo Red "high purity grade"
80028 100MG
EUR 172
Description: Minimum order quantity: 1 unit of 100MG

Amyloid Stain Kit (Congo Red)
AMY-1 1 kit(s)
EUR 165

Amyloid Stain Kit (Congo Red)
AMY-2 1 kit(s)
EUR 109

Congo Red *UltraPure grade* *CAS 573-58-0*
23056 100 mg
EUR 115

thermal paper 112mm/25m
AM112 ea
EUR 24

UPP-110HG Print paper
2140913 1unit
EUR 356

Paper 2.25"x50 feet
B4000-PA 1 PC
EUR 342.53

Blotting Paper, 10X15cm, 100 Sheets
PP3322 1PK, 100UNIT
EUR 105.68

Blotting Paper, 15X15cm, 100 Sheets
PP3323 1PK, 100UNIT
EUR 117.04

Blotting Paper, 20X20cm, 100 Sheets
PP3324 1PK, 100UNIT
EUR 166.4

Weighing Paper, 3X3, 200Sheets/Bag
WT111 1PK, 200UNIT
EUR 57.29

Weighing Paper, 4X4, 100Sheets/Bag
WT112 1PK, 100UNIT
EUR 57.83

Weighing Paper, 6X6, 100Sheets/Bag
WT113 1PK, 100UNIT
EUR 60.44

Beaucage reagent
HY-100951 10mM/1mL
EUR 126

BOP reagent
5-02141 25g Ask for price

BOP reagent
5-02142 100g Ask for price

Bradford reagent
BDE641 100ml
EUR 61.01

BOP reagent
A7015-100000 100 g
EUR 200
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

BOP reagent
A7015-25000 25 g
EUR 113
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

Bluing Reagent
BRT030 30 ml
EUR 60

Bluing Reagent
BRT125 125 ml
EUR 63

Bluing Reagent
BRT3800 1 Gal.
EUR 184

Bluing Reagent
BRT500 500 ml
EUR 76

Bluing Reagent
BRT999 1000 ml
EUR 88

Chymase reagent
30C-CP1129 5 units
EUR 2185
Description: Purified native Human Chymase reagent

Traut's Reagent
2330-1000
EUR 349

Traut's Reagent
2330-500
EUR 207

MTS Reagent
2808-1000
EUR 990

MTS Reagent
2808-250
EUR 365

MTT Reagent
2809-1G
EUR 180

MTT Reagent
2809-5G
EUR 544

BCA Reagent, 16ML
C144-16ML 16ML
EUR 163

Dissociation Reagent, 1ML
X017-1ML 1ML
EUR 109

Dissociation Reagent, 25ML
X017-25ML 25ML
EUR 258

Dissociation Reagent, 5ML
X017-5ML 5ML
EUR 122

Dissociation Reagent, 1ML
X058-1ML 1ML
EUR 73

Dissociation Reagent, 5ML
X058-5ML 5ML
EUR 109

Biolipidure-1002-Reagent
Biolipidure-1002-10 10mL
EUR 196
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

HighGene transfection reagent
RM09014 1000μl
EUR 270

LP4K Transfection Reagent
LP4K 1.0 ml / vial
EUR 304
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types.

n-Heptane Reagent
HC5400 1L
EUR 79

Tri-RNA Reagent
FATRR-001 100ml
EUR 236

Tri-RNA Reagent
FATRR-002 50ml
EUR 176

Tri-RNA Reagent
FATRR-003 450ml
EUR 645

DTT (Cleland's reagent)
DB0058 5g
EUR 84.8

DTNB (Ellman's Reagent)
DB0113 5g
EUR 97.85

Ethyl acetate Reagent
EC4600 1L
EUR 79

TissueDigest Reagent, 20X
T101 10ml
EUR 210

Convoy? Transfection Reagent
1110-1ml
EUR 341

Griess Reagent Kit
30100 1KIT
EUR 149
Description: Minimum order quantity: 1 unit of 1KIT

PhosphoBlocker Blocking Reagent
AKR-103 1L
EUR 328
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

PhosphoBlocker Blocking Reagent
AKR-104 4L
EUR 711
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

EL Transfection Reagent
20-abx098880
  • EUR 384.00
  • EUR 537.00
  • 0.75 ml
  • 1.5 ml

Mycoplasma Prevention Reagent
20-abx098886
  • EUR 425.00
  • EUR 509.00
  • 1 ml
  • 5 ml

Girard's reagent T
20-abx184099
  • EUR 203.00
  • EUR 314.00
  • 100 g
  • 500 g

FcR blocking Reagent
20-abx290024
  • EUR 377.00
  • EUR 516.00
  • 200 tests
  • 400 tests

Detection Reagent A
abx296004-120ul 120 ul
EUR 321

Mycoplasma Prevention Reagent
20-abx298005
  • EUR 203.00
  • EUR 286.00
  • 1 ml
  • 5 ml

Biolipidure-1002-Reagent
Biolipidure-1002-100 100mL
EUR 1223
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-103-Reagent
Biolipidure-103-10 10mL
EUR 196
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-103-Reagent
Biolipidure-103-100 100mL
EUR 1223
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1201-Reagent
Biolipidure-1201-10 10mL
EUR 196
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1201-Reagent
Biolipidure-1201-100 100mL
EUR 1223
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1301-Reagent
Biolipidure-1301-10 10mL
EUR 196
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1301-Reagent
Biolipidure-1301-100 100mL
EUR 1223
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-203-Reagent
Biolipidure-203-10 10mL
EUR 196
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-203-Reagent
Biolipidure-203-100 100mL
EUR 1223
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-206-Reagent
Biolipidure-206-10 10mL
EUR 196
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-206-Reagent
Biolipidure-206-100 100mL
EUR 1223
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-405-Reagent
Biolipidure-405-10 10mL
EUR 196
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-405-Reagent
Biolipidure-405-100 100mL
EUR 1223
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-502-Reagent
Biolipidure-502-10 10mL
EUR 196
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-502-Reagent
Biolipidure-502-100 100mL
EUR 1223
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-702-Reagent
Biolipidure-702-10 10mL
EUR 196
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-702-Reagent
Biolipidure-702-100 100mL
EUR 1223
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-802-Reagent
Biolipidure-802-10 10mL
EUR 196
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-802-Reagent
Biolipidure-802-100 100mL
EUR 1223
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Alcohol, Reagent (70%)
EAS500 500 ml
EUR 79

Alcohol, Reagent (70%)
EAS999 1000 ml
EUR 101

PureFection Transfection Reagent
LV750A-1 1 ml
EUR 359

Biotin reagent (HRP)
65C-CE0202 5 mg
EUR 244
Description: HRP conjugated biotin labelling reagent

BSA (Reagent Grade)
30-AB79 1 kg
EUR 1552
Description: Reagent Grade Bovine Serum Albumin (99% pure)

BSA (Reagent Grade)
30-AB81 200 grams
EUR 476
Description: Reagent Grade Sulphydryl Blocked BSA (99% pure)

HAMA blocking reagent
85R-1001 1 gram
EUR 1974
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA

HAMA blocking reagent
85R-1001P 1 gram
EUR 2190
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA

HAMA blocking reagent
85R-1003 1 gram
EUR 1974
Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests

HAMA blocking reagent
85R-1014 50 mg
EUR 192
Description: HAMA blocking reagent for use in assays specific for clinical false positive samples

HAMA blocking reagent
85R-1025 50 mg
EUR 192
Description: HAMA blocking reagent for use in immunoassays

HAMA blocking reagent
85R-1026 50 mg
EUR 192
Description: HAMA blocking reagent for use in immunoassays

Specimen Preservation Reagent
DA0970 100 test/kit Ask for price

Bradford Dye Reagent
0209R 100 ml
EUR 131

BODIPY-Acetylene Reagent
2594-1
EUR 207

BODIPY-Acetylene Reagent
2594-5
EUR 675

ExFect2000 Transfection Reagent
T202-01 0.5 ml
EUR 227

ExFect2000 Transfection Reagent
T202-02 1 ml
EUR 316

ExFect2000 Transfection Reagent
T202-03 5 ml
EUR 1052

Printer paper for AB-2270, 10/pk
3511065 2unit
EUR 363

Extra paper rolls for MR9600-TP printer
MR9600-PA 1 PC
EUR 86.75

Crimean-Congo hemorrhagic fever virus Nucleoprotein (N)
1-CSB-EP328701CSB
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Crimean-Congo hemorrhagic fever virus Nucleoprotein(N) expressed in E.coli

Crimean-Congo hemorrhagic fever virus PCR kit
PCR-VH060-48R 50T
EUR 823

Crimean-Congo hemorrhagic fever virus PCR kit
PCR-VH060-96R 100T
EUR 1113

Methyl red
MB0627 25g
EUR 61.31

Neutral red
NB0652 25g
EUR 65.66

Phenol red
PB0420 25g
EUR 70.88

Tetrazolium Red
HY-D0714 500mg
EUR 108

Nile Red
HY-D0718 50mg
EUR 124

Trypan red
HY-D0983 5mg
EUR 533

Cresol Red
GT0793-100G 100 g
EUR 78

Cresol Red
GT0793-25G 25 g
EUR 43

Nile Red
GT2331-1G 1 g
EUR 174

Nile Red
GT2331-250MG 250 mg
EUR 87

Chlorophenol Red
GT3722-10G 10 g
EUR 78

Chlorophenol Red
GT3722-25G 25 g
EUR 126

Chlorophenol Red
GT3722-50G 50 g
EUR 198

Chlorophenol Red
GT3722-5G 5 g
EUR 59

Pyrogallol Red
GT7202-10G 10 g
EUR 158

Pyrogallol Red
GT7202-1G 1 g
EUR 54

Alle Beteiligten können von der Umsetzung dieser Empfehlungen profitieren, die darauf abzielen, die Belastungen zu minimieren und sicherzustellen, dass mehr Studienorte und Patienten rechtzeitig Zugang zu klinischen Onkologie-Studien haben. Um eine sinnvolle Wirkung zu erzielen, ist die Annahme und konsequente Umsetzung dieser Empfehlungen in allen Studien, Sponsoren, CROs und Standorten von entscheidender Bedeutung.

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